PCR primers used for detection of F-M04- and F-sial RNAa.
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a = these primers were also used to amplify and clone (for sequence determination) the Australian isotypes of F-M04 and F-sialb = PCR primers (F = forward; R = reverse)c = each primer consists of a binding part (bold) and is fused to a 5′-att-recombination sequence. The latter part of the primer was used for consecutive cloning and sequencing of the amplification product into a Gateway donor vector.
创建时间：
2015-12-02
