Impact of phlebotomy induced anemia on whole retinal transcriptome at P15 and P20 in the rat 50/10 OIR model

Phlebotomy-induced-anemia (PIA), which induces tissue hypoxia and angiogenesis, occurs universally among infants at risk for severe retinopathy of prematurity (ROP). We hypothesized that PIA exacerbates pathologic retinal neovascularization in ROP. Methods: We induced PIA to a hematocrit of 18% among rats undergoing the established 50/10 oxygen-induced retinopathy (OIR) model. Rats were euthanized at P15 and P20, during the avascular and neovascular phases of OIR, respectively. Whole retinal transcriptomes were compared to non-PIA controls. Results: RNA sequencing showed dampened pathways of angiogenesis, inflammation, and neural development in anemic OIR females at P20. Conclusion: PIA dampened transcriptomic pathways central to retinal vascular and neural development in neonatal rats. These data suggest PIA provides a protective effect from ROP.静脉采血诱导贫血（Phlebotomy-induced anemia, PIA）可引发组织缺氧与血管生成，在存在重度早产儿视网膜病变（retinopathy of prematurity, ROP）风险的早产婴儿中普遍发生。本研究假设PIA会加重ROP的病理性视网膜新生血管形成。 方法：本研究在已建立的50/10氧诱导视网膜病变（oxygen-induced retinopathy, OIR）模型大鼠中诱导PIA，使其血细胞比容达到18%。分别于OIR的无血管期和新生血管形成期（即出生后第15天[P15]与第20天[P20]）对大鼠实施安乐死。将全视网膜转录组与非PIA对照组进行比较。 结果：RNA测序显示，出生后第20天的贫血OIR雌性大鼠体内，血管生成、炎症反应及神经发育相关通路均受到抑制。 结论：PIA抑制了新生大鼠视网膜血管与神经发育相关的核心转录组通路。上述数据表明PIA对ROP具有保护作用。