Requirement of hepatic pyruvate carboxylase during fasting, high fat and ketogenic diet

Pyruvate has two major fates upon entry into mitochondria, the oxidative decarboxylation to acetyl-CoA or the biotin-dependent carboxylation to oxaloacetate via pyruvate carboxylase (Pcx). Here we have generated mice with a liver specific knockout of Pcx to understand its role in hepatic mitochondrial metabolism under disparate physiological states including a 24 hour fast, as well as ketogenic and high fat diets. These data ultimately show the requirement of Pcx-mediated anapleorsis in the liver under disparate metabolic conditions. Overall design: RNA-seq on liver tissue from 9wk old male wild-type and Pcx liver-specific knockout mice fasted for 24 hours.