Glycosylation-related gene expression in gastrointestinal cell line HT29 in response to infection by Campylobacter jejuni

HT29 cells were infected with C. jejuni 11168 and the transcriptome of infected and uninfected HT29 were compared by micoarray analysis (Affymetrix). The study focused on glycosylation related genes as these regulate the structures that have a major role to play in pathogen-host cell interaction. This experiment is related to another experiment where the parental cell line (HT29) is replaced by the derivative cell line (E12). E12 cells secrete an adherent mucus layer whereas HT29 cells do not. Two control uninfected HT29 replicates are compared to two C. jejuni (11168) infected HT29 replicates