Neuronal cell labelled with anti MAP2 antibody - sample image for software testing

Hippocampal neurons in culture seeded on glass coverslip. Dendritic extensions are labeled with antibody against MAP2, a protein associated with microtubules and nucleus is labeled with DAPI. The resulting signal give acces to the neuronal cell body and dendrite. Here the axon is not labeled. This picture has been taken on a Leica DMRE microscope with a 40x objective. This is a sample demo to be used to test our automatic segmentation of neuronal cells thanks to the Easy cell shape protocol, available freely on Icy software. You can download the software (PC, Mac or linux) and have access to the full documentation of this solution on http://icy.bioimageanalysis.org/protocol/easy-cell-shape-with-hk-means/ (Publication ID: ICY-77638). It segments in a user friendly mode the cell shape using the hk-mean plugin that applies a N-class thresholding based on a K-Means classification of the image histogram.