Carnivore Diet as a Variant Ketogenic Therapy: The Carnivore Neuroketogenic Framework (CNF) for Epilepsy

This record contains the supplementary materials for the conceptual review titled Carnivore Diet as a Variant Ketogenic Therapy: The Carnivore Neuroketogenic Framework (CNF) for Epilepsy. The materials provide expanded methodological detail, mechanistic pathways, evolutionary background, and a structured research agenda that supports the main manuscript. These supplements are designed to accompany the forthcoming preprint and provide additional clarity for researchers, clinicians, and reviewers. The supplementary materials include: Expanded Methods Appendix Detailed procedures for metabolic assays, continuous glucose monitoring, ketone measurement, amino acid panels, inflammatory markers, mitochondrial assays, EEG protocols, data management, missing data procedures, and quality control thresholds. Key term: SOP integration Mechanistic Pathways Extended explanations of metabolic background, ketone signalling, amino acid flux, inflammation pathways, and ion channel energetics within the CNF model. Key term: Metabolic background Evolutionary Neurometabolic Background Additional detail on hominin trophic level evidence, ancestral ketone utilisation, and neuronal energetics. Key term: Hominin trophic level evidence Proposed Research Agenda A structured set of future research directions including case series, metabolic biomarker studies, EEG investigations, neuroimaging, amino acid trials, inflammatory panels, and mitochondrial function assays. Key term: Carnivore case series Evidence Tiering Notes Definitions and criteria for established evidence, plausible inference, and speculative but mechanistically grounded claims. Key term: Evidence tiering Glossary Definitions of key terms used throughout the CNF framework including neuroketogenic state, bioavailable chemical potential, microglial activation, and GKI. Key term: CNF glossary These materials support the scientific foundation of the CNF model and provide methodological transparency for future replication and extension. They are intended for open science dissemination and long term accessibility through Zenodo.