V4 18SrRNA HTP Metabarcoding of a Brazilian lagoon - Piratininga
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https://www.ncbi.nlm.nih.gov/sra/ERP106790
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资源简介：
Unpublished Results from a HTP metabarcoding for access the eukaryotic biodiversity of the Piratininga Lagoon, Rio de Janeiro, Brazil. Total genome DNA from samples was extracted using CTAB/SDS method. The V4 region of the 18SrRNA were amplified using specific primers. All PCR reactions were carried out with Phusion? High-Fidelity PCR Master Mix (New England Biolabs).The libraries were generated with NEBNext? UltraTM DNA Library Prep Kit for Illumina and quantified via Qubit and Q-PCR, would be analysed by Illumina Hi-seq platform.
创建时间：
2023-08-30
