Identification of formononetin as the active compound of CR-SR in hepatocellular carcinoma treatment: An integrated approach combining network pharmacology and weighted gene co-expression networks

Curcumae Rhizoma and Sparganii Rhizoma (CR-SR) is a classic herbal combination for treating HCC in China. Formononetin is the main active ingredient in CR-SR. To understand the pharmacological properties of formononetin, human hepatoma HepG2 cells were stimulated with or without 625μM formononetin for 72 h. Then, total RNA was extracted for gene expression profile analysis. We performed gene expression profiling analysis using data obtained from RNA-seq of HepG2 cells treated with or without  formononetin.