Regulation of blood-brain barrier by astrocyte through mitochondrial transfer II

In our study, astrocytes were proven to be capable of delivering mitochondria to endothelial cells, and thus regulate the integrity of blood-brain barrier. We utilized RNA seq to investigate the effect of astrocyte-derived mitochondria on endothelial cells. Overall design: Mitochondria were isolated from primary mice astrocytes and were then transplanted to cultured bEnd.3 endothelial cells in vitro. After 24h of co-incubation, bEnd.3 endothelial cells transplanted with or without astrocyte-derived mitochondria were collected for high-throughput RNA seq.