Expression profiling of Chickpea genes differentially regulated during a resistance response to Ascochyta rabiei

Using microarray technology and a set of chickpea (Cicer arietinum L.) unigenes, grasspea (Lathyrus sativus L.) ESTs and lentil (Lens culinaris Med.) resistance gene analogs, the ascochyta blight (Ascochyta rabiei (Pass.) L.) resistance response was studied in four chickpea genotypes, including resistant, moderately resistant, susceptible and wild relative (Cicer echinospermum L.) genotypes. The experimental system minimized environmental effects and was conducted in reference design, where samples from mock-inoculated controls acted as references against post-inoculation samples. Robust data quality was achieved through the use of three biological replicates (including a dye-swap), the inclusion of negative controls, and strict selection criteria for differentially expressed genes including a fold change cutoff determined by self-self hybridizations, Students t test and multiple testing correction (P

运用微阵列技术及一系列鹰嘴豆（Cicer arietinum L.）单基因、草豆（Lathyrus sativus L.）ESTs和菜豆（Lens culinaris Med.）抗性基因同源物，本研究对四种鹰嘴豆基因型（包括抗病、中等抗病、易感及野生近缘种基因型Cicer echinospermum L.）的梭菌叶枯病（Ascochyta rabiei (Pass.) L.）抗性反应进行了探究。实验系统最大限度地减少了环境因素的影响，并在参照设计中实施，其中模拟接种对照组的样品作为接种后样品的参照。通过使用三个生物学重复（包括染料交换）、纳入阴性对照以及严格选择差异表达基因（包括由自我杂交、Student t 检验和多重检验校正确定的倍数变化截止值）等手段，实现了稳健的数据质量。