Expression Characteristics of Genes Hypermethylated and Downregulated in Rat Liver Specific to Non-genotoxic Hepatocarcinogens

The present study examined hypermethylated and downregulated genes specific to carbon tetrachloride (CCl4) by Methyl-Seq analysis combined with expression microarray analysis in the liver of rats treated with CCl4 or N-nitrosodiethylamine (DEN) for 28 days, by excluding those with DEN. Gene expression microarray analysis was performed using total RNA from liver tissue samples of untreated controls, DEN (4 mg/5 mL/kg body weight, dissolved in saline) and CCl4 (100 mg/5 mL/kg body weight, dissolved in corn oil) groups (n = 3/group) after repeated treatment by oral gavage for 28 days.