Transcriptome sequencing of Bactrocera and Zeugodacus fruit flies

Development of novel molecular methods for accurate and economical identification of species has become critical both for pure biological research and in a wide range of applied areas. The most widely used current molecular diagnostic tool, the mitochondrial cytochrome c oxidase subunit 1 gene (CO1), the so-called 'DNA barcode', has been highly criticised and is known to be ineffective at distinguishing species in many groups. To address this, we used transcriptomes and a novel analytical workflow to develop new potential diagnostic markers.