Sox3 expression in neural progenitor differentiation.
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A: Time course of Sox3 gene expression levels by qRT-PCR throughout N2B27 differentiation (normalised to day 4 Sox3 expression, n = 3). B: IHC of SOX3 showing expression at days 2, 4 and 6 of neuronal differentiation. C: GFP expressing cells sorted by FACS within Sox3 null N2B27 differentiated ES cells at days 2, 4 and 7. D: qRT-PCR of Sox1, Sox2, Sox2 and Pax6 expression at day 4 of N2B27 differentiation (n≥4). RQ: relative quantification normalised to β-actin.
创建时间:
2016-02-23



