Distinct T cell functions enable efficient immunoediting and prevent tumor emergence in developing sarcomas
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https://datadryad.org/dataset/doi:10.5061/dryad.v6wwpzh7c
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资源简介：
5’scRNA-seq from sorted live singlet CD45- CD31- TER119- tdTomato+ cells
of KPFRT CreER/Ai65 RagWT, KPFRT CreER/Ai65 Rag KO, and KWT PFRT
CreER/Ai65 RagWT mice was performed with each sample in its own sequencing
lane on an Illumina Novaseq, was processed with Cell Ranger 9.0.0 using
the mm10 mouse genome indices from 10X Genomics. We then used R (v4.4.1)
with Seurat (v5.2.1) for preprocessing and analysis. Cells with greater
than 200 features and less than 10% mitochondrial reads were included. RNA
data was normalized, scaled, and principal components were found. The
functions FindNeighbors, FindClusters, and RunUMAP were then run using
principal components 1-15 and a resolution of 0.5. The packages ggplot2
(v3.5.2), ggpubr (v0.6.0), and dittoseq (v1.16.0) were used for
visualizations.
提供机构：
Dryad
创建时间：
2025-09-11
