Next Generation Sequencing evaluation of DNA mutations in the offspring of male mice exposed to BaP

Understanding the effects of environmental exposures on germline mutation rates has been a decades-long pursuit in genetics. The purpose of this project was to use next-generation sequencing to investigate genome-wide mutations in the offspring of male mice exposed to benzo(a)pyrene (BaP), a common environmental pollutant. We demonstrate that offspring developing from exposed sperm have significantly more de novo single nucleotide variants (1.8-fold increase; P < 0.01) than controls.