The impact of Unc45A silencing on gene expression in the human breast cancer Hs578T (ATCC HTB-126) cell line. The impact of Unc45A silencing on gene expression in the human breast cancer Hs578T (ATCC HTB-126) cell line

The co-chaperone Unc45A is essential for cancer cell growth. We show that Unc45A, a cytoplasmic protein in normal cells, becomes nuclear in cancer cells. We used Microarray to determine the impact of UNC45A on gene expression. We found that Unc45A unctions as a co-activator of the glucocorticoid receptor. In this capacity, it differentially promotes expression of the mitotic kinase NEK7 and other genes possibly through regulation of nuclear receptors. Overall design: Human breast triple negative Hs578T cancer cells were transfected with siRNA against Unc45A or non-targeting siRNA control.