Phosphoproteomic study of HMMR loss in neuroblastoma cells

This is processed dataset from a mass spectrometric analysis of phosphopeptides extracted from KELLY neuroblastoma cells. It compares peptides from cells with wild type HMMR genes and cells where the HMMR is inactivated using CRISPR/Cas9. The analysis aimed to understand how intracellular signaling was altered after the removal of HMMR to understand the potential functional role of HMMR in these tumour cells. the signalling networks in which HMMR was involved in these tumour cells. The are four cell types used: KELLY (parental), KC17 (subject to CRISPR/Cas9 but with intact HMMR genes), KA5 and KA14 (subjected to CRISPR/Cas9 and with out of frame mutations in HMMR, and not expressing the protein). We examined peptides in KA5 and KA14 that show significantly different phosphorylation from those found in the KELLY and KC17. The MS-482 datafile contain several outputs including heatmaps, KSEA analyses plus detailed individual peptide data after processing as described in Thompson, E. M., et al. (2022). FEBS Open Bio. DOI: 10.1002/2211-5463.13418.