Mapping the zebrafish chromatin accessibility landscape by UUATAC-seq

The current understanding of regulatory sequences in the vertebrate genome is still incomplete. By introducing an adapter switching strategy, we developed an ultra-throughput ultra-sensitivity single-nucleus ATAC-seq (UUATAC-seq) protocol that enables construction of high-quality chromatin accessibility landscape from one species in a one-day experiment. By using UUATAC-seq, we mapped open chromatin regions for five representative vertebrate species at the landscape scale. UUATAC-seq was performed across 11 tissues from 4 male and 4 female zebrafish to profile over 90,000 nuclei. Zebrafish tissues were obtained from wild-type D. rerio AB strain.