Antibiotics-induced depletion of rat microbiota induces changes in the expression of host drug-processing genes and pharmacokinetic behaviors of CYPs probe drugs

This study aimed to quantify and compare the mRNA abundance of drug-processing genes (DPGs) in liver and jejunum between wild-type rats and antibiotics-treated rats by using an unbiased quantitative RNA-Seq method. Our results show that antibiotics treatments altered the mRNA expressions of 112 DPGs in the liver and jejunum of rats. The mRNA levels of CYP2A1, CYP2C11, CYP2C13, CYP2D, CYP2E1, and CYP3A of CYP family members were significantly downregulated in antibiotics-treated rats. mRNA profiles of liver and jejunum of wild-type rats and antibiotics-treated rats were generated by RNA-seq, in triplicate, by Illumina NovaSeq 6000.