Additional file 18 of Fine-tuning the metabolic rewiring and adaptation of translational machinery during an epithelial-mesenchymal transition in breast cancer cells
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Additional file 18:. Supplementary Table T4. Translation efficiencies. Polysomal fractionation was done as described (15) with some modifications. Polysomal fractions were not pooled but instead fractions 7-14 for all samples were used individually for study gene expression analysis by qRT-PCRs (3 replicates per fraction, 24 points per sample). Expression was normalized to the pGEMEX-1 RNA spike-in control. Values were calculated from the quantitation cycle (Cq) using the formula relative quantity = 2 Cq(min)-Cq(sample). The inverse of corrected Cq values (corrected Cq-1) for specific monosomes and polysomes fractions are shown on the left. Translation efficiencies with different criterias were determined as the fold change of the polysomes fractions vs the monosomes fractions.
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2020-07-19
