Global gene expression of human iPSC directed differentation towards smooth muscle cells using an Acta2eGFP human iPSC reporter line. Global gene expression of human iPSC directed differentation towards smooth muscle cells using an Acta2eGFP human iPSC reporter line

Here, we generated and differentiated a human induced pluripotent stem cell line with an ACTA2eGFP reporter towards a smooth muscle-like cell that can be purified and expresses characteristic markers of smooth muscle cells. We performed microarray analysis of day 30 ACTA2eGFP+ and ACTA2eGFP- sorted populations, and the transcriptomic profile of the ACTA2eGFP+ cells is reminiscent of an immature or a synthetic smooth muscle cell phenotype. Overall design: We prepared microarray expression profiles comparing sorted day 30 ACTA2eGFP+ and ACTA2eGFP- candidate SMCs from our iPSC-SMC directed differentiation. Biological triplicates of all samples were prepared. Global gene expression in all 15 samples was analyzed by Affymetrix GeneChip Human Gene 2.0 ST arrays.