Lilium formosanum Transcriptome or Gene expression

Identification of MADS-box genes Involved in flower development and floral organ identity by de novo transcriptome sequencing of Formosa lily (Lilium formosanum). To obtain a global overview of the Formosa lily transcriptome and gene activity at nucleotide resolution level, we first extracted total RNA from reproductive tissues including 0.5-1.5 mm floral buds in length. This preparation was for setting up the F_1 transcriptome dataset. Second preparation by extracting total RNA from mature vegetative leaves was conducted to set up the F_2 transcriptome dataset. The mRNA from each sample was isolated, enriched, sheared into smaller fragments, and further reverse-transcribed into cDNA. The different separated cDNA pools from the two preparations were subjected to Illumina HiSeqTM 2000 sequencing, and the resulting.