RawData_NTA_FTLD

Nanoparticle Tracking Analysis (NTA) data are obtained from EVs isolated from 172 human plasma samples, from patients with sporadic FTLD (n=40), with FTLD due to C9orf72 expansion (n = 9 C9orf72 intermediate expansion carriers; n = 31 C9orf72 pathological expansion carriers), with FTLD due to pathogenic mutations in GRN (n = 46 heterozygous mutation carriers), with NCL due to pathogenic mutation in GRN (n= 3 GRN homozygous null mutation carriers) and subjects with normal cognitive function, as control group (n = 43). Moreover, a negative control was included in the analysis (ctrl_neg). Samples were analyzed with Nano-Sight NS300 Instrument (Malvern, Worchestershire, UK). For each sample, 5 video of 60 seconds duration were recorded and data were processed using NanoSight NTA Software 3.2 (Malvern, Worchestershire, UK). Correspondence_RawData table shows sample code, category as diagnostic group, age and the corresponding raw file name. Raw data of each sample analyzed consists of .csv file generated by Nano-Sight NS300 Instrument. File contains information about EVs concentration (particles/ml, particles/frame), size (mean, mode, SD) and size distribution (D10, D50 and D90). Representative video of each main diagnostic group are available in the NTA_Video folder.

纳米颗粒追踪分析（Nanoparticle Tracking Analysis，简称 NTA）数据源自从 172 份人血浆样本中分离出的外泌体（EVs）。这些样本来自患有散发性进行性前额叶痴呆症（FTLD，n=40）的患者，因 C9orf72 扩增导致的 FTLD 患者（n = 9 C9orf72 中间型扩增携带者；n = 31 C9orf72 病理性扩增携带者），因 GRN 基因中的致病性突变导致的 FTLD 患者（n = 46 杂合突变携带者），以及因 GRN 基因中的致病性突变导致的 NCL 患者（n= 3 GRN 同型纯合子 null 突变携带者）。此外，作为对照组（n = 43），还纳入了认知功能正常的受试者。分析中还包括了一个阴性对照（ctrl_neg）。样本使用 Nano-Sight NS300 仪器（Malvern，Worcestershire，英国）进行分析。对于每个样本，记录了 5 段各 60 秒的视频，并使用 Nano-Sight NTA 软件 3.2（Malvern，Worcestershire，英国）进行处理。'Correspondence_RawData' 表显示了样本代码、诊断组别、年龄以及相应的原始文件名。每个样本分析的原始数据包括由 Nano-Sight NS300 仪器生成的 .csv 文件。文件包含关于外泌体浓度（颗粒/ml，颗粒/帧）、尺寸（平均值、众数、标准差）以及尺寸分布（D10、D50 和 D90）的信息。NTA_Video 文件夹中提供了每个主要诊断组的代表性视频。