Comparative RNA-seq analysis of CD146? pericyte transcriptomes from wild-type and mdx mice

The project aimed to investigate differences in the gene expression profile of pericytes expressing the CD146 marker (CD146+ pericytes) isolated from the skeletal muscles of wild-type (WT) mice and mdx mice, which serve as a fundamental research model for Duchenne muscular dystrophy. The aim was to understand the differences between two cell types and their potential role in the disease progression. Pericytes were isolated from the skeletal muscles of WT and mdx mice and subsequently enriched for the CD146+ population using magnetic column sorting. After five days of in vitro culture, cells were harvested, and total RNA was extracted. Next-generation sequencing (NGS) was performed at the Next Generation Sequencing Core Facility, Center for New Technologies.