SARS-CoV-2 ncrRNA interactome

A deep understanding of SARS-CoV-2-host interactions is crucial to developing effective therapeutics and addressing the threat of emerging coronavirus. The role of non-coding regions of viral RNA (ncrRNAs) has not been systematically scrutinized. We developed a method using MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) to systematically map the interactome of SARS-CoV-2 ncrRNA in Calu-3, Huh7, and HEK293T cells. Integration of the results defined the core ncrRNA-host protein interactomes.深入解析严重急性呼吸综合征冠状病毒2（SARS-CoV-2）与宿主的相互作用，对于开发高效治疗手段、应对新兴冠状病毒的威胁具有关键意义。当前，病毒RNA非编码区（ncrRNAs）的功能尚未得到系统性的深入研究。我们开发了一种结合MS2亲和纯化与液相色谱-质谱联用（MAMS）的实验方法，可在Calu-3、Huh7及HEK293T细胞中系统性绘制SARS-CoV-2非编码RNA的宿主互作组。整合上述实验结果，最终明确了核心的病毒非编码RNA-宿主蛋白互作组。