Genome wide screening for binding target of ELF3 transcription factor in biliary epithrial cell line HBDEC2

ELF3, a member of E26 transformation-specific transcription factors, is inactivated in a range of cancers including biliary tract cancer. We performed chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-Seq) to identify direct ELF3 target genes. The number of uniquely mapped reads identified by ChIP-Seq was 109.9 M for the ELF3 binding site and 59.7 M for the control input DNA. These read data were evaluated with MACS 1.4, and 13,960 peaks were identified throughout the genome with a false discovery rate (FDR) of < 0.1. ELF3 ChIP-Seq was performed on ELF3 gene-knockout HBDEC2 cells with ELF3 tet-ON system.