Effect of EDC exposure on differentiation of iPSC derived stem cell islets

This project investigates the impact enodcrine disrupting chemicals (EDCs) on differentiation toward stem cell derived islets. RNA sequencing (RNA-seq) was performed on EDCs treated and non-treated islets differentiated from iPSCs to SC-islets. By comparing the transcriptomic profiles of these islets, the study aims to uncover molecular mechanisms affected by EDCs treatment, and the impact on SC-islet fate. Overall design: iPSCs were differenitated toward ST-islet fate using a 7 stage step wise protocol. At Endocrine progenitor stage the each well of aggreagtes were split in 2 wells, one for treatment and one for control. For 14 days the treatment were treated with a 1 mM mix of bisphenol A, bisphenol S and trans-nonachlor. The SC-islets were harvested at day 38 of the protocol, 7 days after end of treatment.