Dataset of "Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness"

Dataset_1 Data from functional imaging in the VPM using type II eFOV-microendoscopes before and after the removal of the corrective microlens in awake head-restrained mice. - dataset1_description.csv: description of the t-series containing the mouse ID, the type of lens used and the imaging parameters. - TS####.mat: mat-file containing a structure with imaging data - data.A is a num_pixels*num_ROIs array containing the spatial footprints of the ROIs. Each column corresponds to a single ROI and contains positive values for pixels belonging to the corresponding ROI. - data.F_raw is a num_ROIs*num_frames array containing the raw fluorescence activity of the ROIs. Each row corresponds to a single ROI. - data.C_d is a num_ROIs*num_frames array containing the normalized DF_F activity of the ROIs. Each row corresponds to a single ROI. Dataset _2: Data from functional imaging in the VPM using type II eFOV-microendoscopes with the corrective microlens in awake head-restrained mice. Brief dataset description: - TSeries-########-####-####_Mouse_####.mat: mat-file containing a structure with imaging data - data.A is a num_pixels*num_ROIs array containing the spatial footprints of the ROIs. Each column corresponds to a single ROI and contains positive values for pixels belonging to the corresponding ROI. - data.F_raw is a num_ROIs*num_frames array containing the raw fluorescence activity of the ROIs. Each row corresponds to a single ROI. - data.C_d is a num_ROIs*num_frames array containing the normalized DF_F activity of the ROIs. Each row corresponds to a single ROI. Dataset_3: Auxiliary data to generate synthetic t-series. For a complete description of their use, see https://github.com/moni90/eFOV_microendoscopes_sim Dataset_4 Zemax files for optical simulations. The Zemax data related to the GRIN rods are provided by GRINTECH and are based on refractive index profiles of standard GRINTECH’s 0.25 pitch rod lenses, which are optimized for optimum on-axis spherical aberration. Dataset_5 z-stacks of PSF measurements. Dataset_6 Confocal images of labeling of cortico-thalamic and thalamo-cortical connectivity.数据集1： 针对清醒头部固定小鼠，在移除校正微透镜前后，使用II型eFOV微型内窥镜（type II eFOV-microendoscopes）对腹后内侧丘脑核（ventral posteromedial nucleus，VPM）进行功能成像得到的数据。 - dataset1_description.csv：包含t序列的描述信息，内容涵盖小鼠编号、所用透镜类型及成像参数。 - TS####.mat：包含成像数据结构体的MATLAB数据文件 - data.A：为num_pixels×num_ROIs数组，存储感兴趣区域（Region of Interest，ROI）的空间足迹，每一列对应一个独立ROI，其中属于该ROI的像素取值为正。 - data.F_raw：为num_ROIs×num_frames数组，存储各ROI的原始荧光活动信号，每一行对应一个独立ROI。 - data.C_d：为num_ROIs×num_frames数组，存储各ROI的归一化ΔF/F活动信号，每一行对应一个独立ROI。 数据集2： 针对清醒头部固定小鼠，使用带有校正微透镜的II型eFOV微型内窥镜对腹后内侧丘脑核（VPM）进行功能成像得到的数据。 简要数据集说明： - TSeries-########-####-####_Mouse_####.mat：包含成像数据结构体的MATLAB数据文件 - data.A：为num_pixels×num_ROIs数组，存储ROI的空间足迹，每一列对应一个独立ROI，其中属于该ROI的像素取值为正。 - data.F_raw：为num_ROIs×num_frames数组，存储各ROI的原始荧光活动信号，每一行对应一个独立ROI。 - data.C_d：为num_ROIs×num_frames数组，存储各ROI的归一化ΔF/F活动信号，每一行对应一个独立ROI。 数据集3： 用于生成合成t序列的辅助数据。如需了解其完整使用方法，请访问：https://github.com/moni90/eFOV_microendoscopes_sim 数据集4： 用于光学仿真的Zemax文件。与自聚焦透镜棒（GRIN rods）相关的Zemax数据由GRINTECH公司提供，基于该公司标准0.25节距棒状透镜的折射率分布制备，该透镜针对最优轴上球差进行了优化。 数据集5： 点扩散函数（Point Spread Function，PSF）的Z轴堆叠成像数据。 数据集6： 标记皮层丘脑与丘脑皮层神经连接的共聚焦显微镜图像。