Single cell RNA sequencing of omental tumors or ascites from KPCA syngeneic cancer cell lines

The KPCA ovarian cancer cell line was generated by S. Iyer in the Weinberg lab at the Whitehead Institute (MIT) (S. Iyer et al., 2020). Multiple clones derived from the KPCA cells were established, presenting different level of sensitivity to immunotherapy. To investigate the tumor microenvironment in this syngeneic mouse model, we implanted the different KPCA clones (3.5 × 10^6 cells) into the peritoneal cavity. Ten days after grafting, we performed single-cell RNA sequencing on harvested tumors and cells from the peritoneal fluid. Overall design: Single-cell RNA sequencing of KPCA.A, KPCA.B, and KPCA.C tumors and ascites (N=4 pooled tumors per genotype, 5000 cells)