Data_Sheet_1_Biological Characteristics and Genetic Analysis of a Highly Pathogenic Proteus Mirabilis Strain Isolated From Dogs in China.docx

To evaluate the antimicrobial resistance and virulence gene characteristics of highly pathogenic Proteus mirabilis. In this study, we isolated P. mirabilis CC15031 from diarrhea dogs in China, tested the median lethal dose (LD50), and measured the minimum inhibitory concentration (MIC) of 10 different antibiotics commonly used in veterinary clinic. Meanwhile, we presented the complete genome sequence annotations to analyze the virulence and resistance formation mechanism. The results showed that the CC15031 presented relatively potent pathogenicity in mice (LD50 = 0.57 × 106 CFU) and exhibited a high degree of resistance to all the tested antimicrobial agents. The CC15031 genome of 4,031,742 bp with 3,745 predicted genes had an average gene length of 917 bp and 38.99% guanine-cytosine content. A new variant of an integrative and conjugative element with a type IV secretion system (217,446 bp) conferring multidrug resistance was identified and characterized by structural analysis in CC15031. These data provide a foundation for understanding the genomic features and antimicrobial resistance mechanisms of this pathogen.

为评估高致病性变形菌属奇异变形菌的抗菌耐药性和毒力基因特征，本研究从中国腹泻犬中分离出奇异变形菌CC15031，检测了其中等致死剂量（LD50），并测量了10种在兽医诊所中常用抗生素的最小抑菌浓度（MIC）。同时，我们展示了完整的基因组序列注释，以分析毒力和耐药性形成机制。结果显示，CC15031在小鼠中表现出相对较强的致病性（LD50 = 0.57 × 10^6 CFU），并对所有测试的抗菌剂具有高度耐药性。CC15031的基因组（4,031,742 bp）含有3,745个预测基因，平均基因长度为917 bp，鸟嘌呤-胞嘧啶含量为38.99%。通过结构分析在CC15031中鉴定并表征了一种新的整合与接合元件变异体，该变异体携带有IV型分泌系统（217,446 bp），赋予多重耐药性。这些数据为理解该病原体的基因组特征和抗菌耐药性机制奠定了基础。