code and datasets for "GP73 reinforces cytotoxic T-cell function by regulating HIF-1α and increasing antitumor efficacy"

We utilized T-cell-specific GP73 knockout mice to establish MC38 and B16 tumor models to investigate the impact of GP73-deficient T cells on tumor growth. Single-cell sequencing was subsequently employed to classify tumor-infiltrating immune cells and assess changes in cytokines and metabolic genes. Through RNA sequencing, real-time quantitative PCR, western blotting, flow cytometry, seahorse analysis, glucose uptake, and lactate secretion assays, we explored how GP73 regulates HIF-1α to influence T-cell antitumor functionality. Furthermore, we established adoptive transfer experiments to study the ability of GP73-overexpressing T cells to combat tumors. Clinical tumor patient blood samples were collected to assess the relationship between immunotherapy efficacy and T-cell GP73 levels.