Raw data for Fig 7

<b>Contents:</b><br>These data relate to Fig.7 of Sampattavanich et al., Cell Systems (2018). Refer to accompanying Git repository for scripts used for Fig.7. <br><b>To use this data with our Git Repository:</b>Placed these in the /rawdata/fixedcell folder.<br>Each cell line has three .MAT files for three proteins: FoxO3, ERK and AKT. Each single_Protein variable has the dimension of TimePoint x Row x Column. <br><i>Raw Index</i> indicates growth factor types, each at 100 ng/mL: (1)EGF (2)IGF1 (3)FGF1 (4)HRG (5)HGF (6)EPR (7)BTC (8)Non-stimulated control<br><i>Column Index</i> indicates drug pre-treatment condition: (1)DMSO control (2)AKTi(10uM) (3)MEKi(10uM) (4)AKTi(10uM)+MEKi(10uM)<br><i><br></i><i>Timepoint Index</i> varies between cell lines<i>184A1</i>: 08-19-2013 (result 08-22-2013) including 13-timepoints: 0, 5, 10, 15, 20, 30, 45, 60, 90, 120, 180, 300, 480 minutes<br><i>MCF10A</i>: 11-26-2013 (result 11-29-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.<br><i>SKBR3</i>: 10-22-2013 (result 10-25-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.<br><i>BT-20</i>: 10-22-2013 (result 10-25-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.<br><i>MCF7</i>: 11-05-2013 (result 11-09-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.<br><i>T47D</i>: 11-26-2013 (result 11-29-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.<br><i>Hs578T</i>: 11-05-2013 (result 11-08-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.<br><i>MDA231</i>: 11-26-2013 (result 11-29-2013) including 8 time points:0,0:15,0:30,1,1:30,2,3,4HRs.<br><i>HCC1806</i>: 12-17-2013 (result 12-20-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.<br><b>Experimental Setup:</b><br>Cells were serum starved for 6 hours, and were then pre-treated with different inhibitor conditions for 1 hour. Cells were stimulated with different growth factor types, and fixed at various timepoints. Fixed cells were then stained with FoxO3, phospho-AKT, and phospho-ERK using standard immunofluorescent staining.