Comparison of VNAR display libraries with different expression systems

We analyzed the potential biases of phage display and yeast display in VNAR development. Construction and enrichment of VNAR displaying libraries is usually utilized either phage or yeast display technologies, but no reports have addressed the differences in inherent bias between phage and yeast displays and their impact on the outcomes of VNAR acquisition. We constructed phage display libraries and yeast display libraries using RNA from an immunized Japanese topeshark. We analyzed the diversity of VNAR display libraries using next-generation sequencing in order to comprehensively understand VNAR sequence diversities in the libraries before and after biopanning and to evaluate clonal enrichment during biopanning.