Electron Microscopy Data Bank

Cryo-electron microscopy reconstruction methods are uniquely able to reveal structures of many important macromolecules and macromolecular complexes. The Electron Microscopy Data Bank (EMDB) is a public repository for electron microscopy density maps of macromolecular complexes and subcellular structures. It covers a variety of techniques, including single-particle analysis, electron tomography, and electron (2D) crystallography. The EMDB was founded at EBI in 2002, under the leadership of Kim Henrick. Since 2007 it has been operated jointly by the PDBe, and the Research Collaboratory for Structural Bioinformatics (RCSB PDB) as a part of EMDataBank which is funded by a joint NIH grant to PDBe, the RCSB and the National Center for Macromolecular Imaging (NCMI).冷冻电子显微镜（cryo-electron microscopy）重构技术具备独特的技术优势，能够揭示诸多重要生物大分子与大分子复合物的三维结构。电子显微镜数据库（Electron Microscopy Data Bank, EMDB）是面向大分子复合物及亚细胞结构电子显微镜密度图的公共存储库，涵盖单颗粒分析、电子断层成像、电子（2D）晶体学等多种电子显微技术手段。EMDB于2002年在欧洲生物信息研究所（European Bioinformatics Institute, EBI）由Kim Henrick牵头创立；自2007年起，该库由欧洲蛋白质数据库（Protein Data Bank Europe, PDBe）与结构生物信息学研究合作组织（Research Collaboratory for Structural Bioinformatics, RCSB PDB）联合运营，作为EMDataBank的组成部分，其运营经费由一项面向PDBe、RCSB PDB及美国国家大分子成像中心（National Center for Macromolecular Imaging, NCMI）的联合美国国立卫生研究院（National Institutes of Health, NIH）拨款提供支持。

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背景与挑战

背景概述

Electron Microscopy Data Bank (EMDB) 是一个公共存储库，专门用于存储冷冻电子显微镜（cryoEM）下的大分子复合物和亚细胞结构的体积数据与代表性断层扫描图。它涵盖多种技术，如单颗粒分析、螺旋重建、电子断层扫描、亚断层扫描平均和电子晶体学。截至2026年6月3日，该数据库已包含58166个条目。