Eed, a member of the Polycomb group, is required for nephron differentiation and the maintenance of nephron progenitor cells

We used R26R-tdTomato reporter mice activated by the Six2-TGC transgene to isolate cell populations co-expressing GFP and tdTomato by FACS from kidneys of control P0 mice. We observed two distinct populations of cells from control kidneys based on lower or higher tdTomato expression along with high GFP-expression from the Six2-TGC transgene. RNASeq experiment was conducted to analyze the transcriptome of these populations Examination of 2 different populations of cells derived from Six2-expressing kidney by RNA-seq.