LncRNA MSAR stabilizes c-Myc to promote pancreatic cancer malignancy

We identify a collection of candidate lncRNAs interacted with c-Myc in pancreatic cancer and show for the first time that an lncRNA among them, which is named as c-Myc-stabilizing and activating RNA (MSAR), promotes pancreatic cancer proliferation and metastasis through c-Myc. MSAR enhances the interaction of c-Myc and SNIP1. These findings shed light on the pro-tumorigenic role of MSAR and might provide a novel therapeutic strategy for indirect targeting c-Myc in PDAC. Overall design: We identified lncRNAs interacted with c-Myc protein in PANC-1 cell lines using RNA immunoprecipitation sequencing (RIP-Seq) experiment. To explore the molecular mechanism underlying the effects of MSAR on PDAC cell phenotypes, we performed RNA-seq analysis in MSAR-silenced and their control PANC-1 cells. We further performed cross-linking and immunoprecipitation sequencing (CLIP-seq) of SNIP1 to explore the mechanism underlying the interaction of SNIP1 and MSAR. Comparative gene expression profiling analysis of RNA-seq data in MSAR-overexpression and their control PANC-1 cells.