miRNA expression in tracheal aspirates [ICS-BPD vs. ICS-BPD-PH]. miRNA expression in tracheal aspirates [ICS-BPD vs. ICS-BPD-PH]

Tracheal aspirate (TAs) samples were collected from intubated preterm infants with hemodynamically significant intracardiac shunt (ICS), and a diagnosis of ICS-BPD/ICS-BPD-PH. 36 TA samples were analyzed. Small RNAs were extracted and the expression miRNAs was detected with PCR arrays. Overall design: We extracted RNA from 0.5 mL of tracheal aspirates from premature neonates using the Plasma/Serum RNA Purification Kit (Norgen). The expression of 1,066 human miRNAs was quantified using the Human miRNome miScript® miRNA PCR Array (QIAGEN, MIHS-3216Z). Results (Ct values) were normalized to global means, and fold changes were calculated using the 2-ΔΔCT method.