Transcriptomic analysis of tumor infiltrating CD8+ T cells during PDAC progression

CD8+ T cells are master effectors of anti-tumor immunity and their presence at tumor sites correlates with favorable outcomes. However, metabolic constraints imposed by the tumormicroenvironment can dampen their ability to control tumor progression. We will analyze the transcriptional profile of CD8+ T cells infiltrating murine tumors during progression in a pre-cinical model pancreatic ductal adenocarcinoma (PDA). Overall design: Total RNA extraction from CD45+CD3+CD8+ T cells sorted from blood or pancreas of WT B6 mice or KC mice at different time points (4, 13, 20, 0r 30 weeks after birth).