Escherichia coli RNA polymerase unwinding intermediate (I1d) at the lambda PR promoter

Escherichia coli RNA polymerase unwinding intermediate (I1d) at the lambda PR promoter Descriptor: (3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... Authors: Darst, S.A, Saecker, R.M, Mueller, A.U. Deposit date: 2023-08-02 Release date: 2024-07-03 Last modified: 2025-05-21 Method: ELECTRON MICROSCOPY (2.9 Å) Cite: Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy. Nat.Struct.Mol.Biol., 31, 2024大肠杆菌RNA聚合酶在λ PR启动子处的解旋中间体（I1d）。描述符：(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-二甲基-17-[(2R)-戊烷-2-基]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-十四氢-1H-环戊并[a]菲-3,7,12-三醇、DNA依赖的RNA聚合酶（DNA-directed RNA polymerase）α亚基、DNA依赖的RNA聚合酶β亚基……。作者：Darst, S.A.、Saecker, R.M.、Mueller, A.U.。存档日期：2023-08-02；发布日期：2024-07-03；最后修改日期：2025-05-21。实验方法：电子显微镜（分辨率2.9 Å）。引用文献：通过时间分辨冷冻电子显微镜可视化细菌RNA聚合酶启动子解旋的早期中间体。《自然-结构与分子生物学》（Nat.Struct.Mol.Biol.），第31卷，2024年。