Figure 3A

<b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.