Table S3.pdf

Table S3. NO2- production by Methylocystis sp. strain SC2 in response to increasing NH4+ concentrations. Note: SC2 cells were incubated in 120-ml serum bottles under a headspace CH4:air mixing ratio of 20:80 (v/v) at 25 ℃, with shaking at 130 rpm. Samples (1 ml) for measurement of NO2- were collected at 5 (30 mM, 50 mM NH4+) or 6 (1 mM, 10 mM, and 75 mM NH4+) time points. NO2- was measured by the Griess Reagent System (Promega Corporation, Madison, WI). Significant difference between comparison groups were calculated using one-way ANOVA Bonferroni t-test: *p-value≤0.05, **p-value≤0.01, ***p-value≤0.001 [comparing the same incubation time point across the increasing NH4+ concentrations (10 mM, 30 mM, 50 mM, 75 mM NH4+) relative to the control treatment (1 mM NH4+)]; and ǂp-value≤0.05, ǂǂp-value≤0.01, ǂǂǂp-value≤0.001 [comparing the same NH4+ treatment over incubation time in relation to time point 1]. See Fig. 5 for the graphical presentation of NO2- production over the whole incubation period.