Biparental contributions of the H2A.B histone variant control embryonic development in mice

H2A.B KO were generated by CRISPR-editing in B6;SJLF1/J mouse zygotes which were backcrossed to C57BL/6, haploid spermatids were isolated using Hoechst dye, according to Bastos et al., Cytometry Part A, 2005 We used Automated Cleavage Under Targets and Release Using Nuclease (AutoCUT&RUN), a chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA sequencing.