DNA microarray analysis using jejunum from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

To understand the molecular mechanism by which IIAEK ameliorates hepatic and intestinal cholesterol metabolism, we performed DNA microarray analysis using liver, duodenal, and jejunal samples from the control and IIAEK groups of WT or IAP KO mice. We found that 3503 transcripts were identified in the jejunum of WT mice [WT, Control (WC) vs. WT, IIAEK (WI)] and 1388 in IAP-KO mice [IAP-KO, Control (KOC) vs. IAP-KO, IIAEK (KOI)] ( ≥1.2 fold-change, p < 0.05). Seven week-old wild type (WT) and IAP knockout (IAP-KO) (Akp3-/-) mice were administered orally the pentapeptide IIAEK (Ile-Ile-Ala-Glu-Lys) (600 mg/kg B.W./d) once a day for 14 d. After fasting for 8 h, the jejunum was collected from the mice for DNA microarray analyses (n=3/group).