eDNA metabarcoding of seawater plankton across an environmental gradient in Lake Shihwa, South Korea (18S rRNA and COI)

This BioProject contains demultiplexed Illumina amplicon sequencing reads (eDNA metabarcoding) generated from surface seawater samples collected in Lake Shihwa, South Korea. Samples were collected across eight stations (two exterior: SH1-SH2; six interior: SH3-SH8) along a strong environmental gradient and filtered (2 L per station) using Sterivex 0.22 um enclosed filters. Two genetic markers were amplified to profile eukaryotic plankton communities: COI and 18S rRNA. Libraries were prepared with Nextera indexing and sequenced on an Illumina MiSeq platform using 2 x 300 bp paired-end reads. The data are provided as demultiplexed FASTQ files for downstream community and network/modularity analyses.