Sternaspis scutata µCT scan

µCT scan of a specimen of Sternaspis scutata stained with PTA for one week. Scan and reconstruction were obtained with the following settings:

[System]
Scanner=SkyScan1272
Instrument S/N=15H09112
Software Version=1.1.10
Source Type=HAMAMATSU_L11871_20
Camera Type=XIMEA xiRAY16
Camera Pixel Size (µm)=7.4
Camera X/Y Ratio=1.0058

[Acquisition]
Number Of Files=  961
Number Of Rows= 1640
Number Of Columns= 2452
Partial Width=OFF
Image crop origin X=0
Image crop origin Y=0
Camera binning=2x2
Image Rotation=-0.00100
Optical Axis (line)=  890
Camera to Source (mm)=174.64085
Object to Source (mm)=82.59950
Source Voltage (kV)=  70
Source Current (µA)= 142
Image Pixel Size (µm)=6.999924
Scaled Image Pixel Size (µm)=6.999924
Image Format=TIFF
Depth (bits)=16
Reference Intensity=57000
Exposure (ms)=1238
Rotation Step (deg)=0.200
Use 360 Rotation=NO
Scanning position=11.719 mm
Frame Averaging=ON (4)
Random Movement=OFF (15)
Flat Field Correction=ON
FF updating interval=120
Geometrical Correction=ON
Filter=Al 0.5mm
Gantry direction=CC
Rotation Direction=CC
Type of Detector Motion=STEP AND SHOOT
Scanning Trajectory=ROUND
Number Of Horizontal Offset Positions=1
Number of connected scans=3
Current scan number=1
Number of lines to be reconstructed=1036
Scan duration=1h:40m:36s
Maximum vertical TS=5.0

[Reconstruction]
Reconstruction Program=NRecon
Program Version=Version: 1.7.1.0
Reconstruction engine=InstaRecon
Engine version=Version: 2.0.3.7
Reconstruction from batch=Yes
Postalignment=10.00
Connected Reconstruction (parts)=3
Sub-scan post alignment [0]=10.000000
Sub-scan post alignment [1]=10.000000
Sub-scan post alignment [2]=10.000000
Sub-scan scan length [0]=1035
Sub-scan scan length [1]=1035
Sub-scan scan length [2]=1036
Used extra shift in X per scan(micron)= 0.000  -1.993  -4.803 
Used extra shift in Y per scan(micron)= 0.000  -25.239  -44.091 
First Section=63
Last Section=3495
Reconstruction duration per slice (seconds)=0.349107
Total reconstruction time (2071 slices) in seconds=723.000000
Section to Section Step=1
Sections Count=3433
Result File Type=TIF
Result File Header Length (bytes)=12
Result Image Width (pixels)=2152
Result Image Height (pixels)=2056
Pixel Size (µm)=6.99992
Reconstruction Angular Range (deg)=192.20
Use 180+=OFF
Angular Step (deg)=0.2000
Smoothing=3
Smoothing kernel=2 (Gaussian)
Ring Artifact Correction=20
Draw Scales=OFF
Object Bigger than FOV=OFF
Reconstruction from ROI=ON
ROI Top (pixels)=2282
ROI Bottom (pixels)=223
ROI Left (pixels)=214
ROI Right (pixels)=2369
ROI reference length=2452
Filter cutoff relative to Nyquist frequency=100
Filter type=0
Filter type description=Hamming (Alpha=0.54)
Undersampling factor=1
Threshold for defect pixel mask (%)=0
Beam Hardening Correction (%)=54
CS Static Rotation (deg)=78.89
Minimum for CS to Image Conversion=0.003750
Maximum for CS to Image Conversion=0.105554
HU Calibration=OFF
BMP LUT=0
Cone-beam Angle Horiz.(deg)=11.863247
Cone-beam Angle Vert.(deg)=7.950323
Automatic matching in Z=50
Automatic matching in X/Y=50
Automatic matching in rotation=0.000000
Automatic fusion=1

Data size has been reduced by conversion from 16-bit to 8-bit and decreasing the scale of the images.