RNA-seq analysis of transcriptomes of WT and Foxp3∆2 Treg cells in colon lamina propria

We performed RNA-seq analysis of Tregs isolated from Foxp3RFP/∆2Foxp3GFP-cre female mice. CD4+GFP+RFP+ (WT Tregs) and CD4+GFP+RFP- (Foxp3∆2 Tregs) cells were sorted from colon lamina propria to a typical purity of >95%. In order to obtain enough RNA for sequencing, we combined the cells sortd from the cLP of three mice for library construction. Genes that were upregulated (532 genes) and downregulated (497 genes) in Foxp3∆2 Tregs Transcriptional profiles of WT and Foxp3∆2 Treg cells in colon lamina propria