Identification, visualization and clonal analysis of intestinal stem cells in fish

<b>Movie 1: The rostral part of the medaka intestinal tract. </b> A 3D view of X-ray data starting from the buccal cavity and going through the mid-gut reveals distinct topographical regions along the rostro-­‐caudal axis. <b> </b> <b>Movie 2: The caudal part of the medaka intestinal tract. </b> A 3D view of X-ray data from the hind-gut indicating the narrower passage in the caudal part of the intestine with smaller folds. <b>Movie 3: The Gaudí</b><b><i>RS</i></b><b><i>Gubiquitin</i>:^ERT2Cre (10dpi) </b> MuVi-Spim 3D visualization of a gut segment at 10days after induction shows already labeling of strings of clonally related groups representing the stem cells and their descendants (green). <b>Movie 4: The Gaudí</b><b><i>RS</i></b><b><i>Gubiquitin</i>:^ERT2Cre (30dpi) </b> MuVi-Spim 3D visualization of a gut segment at 30 days after induction showing intestinal folds that are either broadly labelled by H2B-­‐ EGFP (green), or not at all (grey) indicating longitudinal expansion of the clones. <b> </b> <b>Movie 5: The Gaudí</b><b><i>RS</i></b><b><i>Gubiquitin</i>:^ERT2Cre (150dpi)</b> MuVi-Spim 3D visualization of a gut segment at 150 days after induction. Two large clones (in green) established by symmetric division and neutral drift. <b>Movie 6: The Gaudí<i>^Lx</i></b><b><i>BB</i></b><b><i>W</i> hsp70:nlsCRE (90dpi)</b> MuVi-Spim 3D visualization of a gut segment 90 days after induction shows that stochastically triggered clonal multi-color labeling results in adjacent clones of different sizes discernible by different fluorescent proteins demonstrated in this movie by yellow, green, blue and purple color.<b></b>