VEGF related gene expression signatures of breast cancer cells

To further development of our gene expression approach, we have employed whole genome microarray expression profiling to identify genes related to VEGF in MDA-MB-231 breast cancer cell line. Total RNA of MDA-MB-231, VEGF-knocked out MDA-MB-231, recombinant VEGF(rhVEGF) treated VEGF-knocked out MDA-MB-231, soluble NRP1 overexpressed MDA-MB-231 cells were used. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agileng Technologies, Santa Clara, CA). VEGF was knocked out using CRISPR/Cas9 system. Soluble NRP1 works as VEGF-TRAP. Cell were cultured for 48 hours before RNA extraction. rhVEGF treatment was done for 48 hours.