RAD-seq of AMPRIL (Arabidopsis multiparent RIL population). AMPRIL RAD-seq

The eight AMPRIL founder accessions (An-1, C24, Col-0, Cvi-0, Eri-1, Kyo, Ler and Sha) were selected across the entire geographic distribution of A. thaliana. The first release of the AMPRIL population (AMPIRL I) contained six subpopulations (referred to as ABBA, ACCA, ADDA, BCCB, BDDB, CDDC) which were created from bi-directional diallel intercrossings between four hybrids (A: Col-0 x Kyo, B: Cvi-0 x Sha, C: Eri-1 x An-1, D: Ler x C24) and the subsequent selfing to the F4 generation by single-seed descent. Here we present the extension of the AMPRIL population with six new subpopulations referred to as EFFE, EGGE, EHHE, FGGF, FHHF and GHHG (E: Col-0 x Cvi-0, F: Sha x Kyo, G: Ler x An-1, H: Eri-1 x C24) based on a different diallel intercrossing scheme and selfing of the recombinant genomes until to the F6 generation. Each subpopulation consists of approximately 90 individuals which are recombinants of four founders. In total, 992 RILs from all twelve subpopulations were sequenced using RAD-seq and genotyped with ~2 million high-quality SNP markers