Time-Series Annotated Dataset of Spore Bud Morphological Development

We present a curated dataset comprising 4,414 original microscopy images and their corresponding manually annotated labels, documenting the morphological development of fungal spore buds. The original images were obtained from the publicly accessible Cell Image Library (CIL) and systematically organized into a time-series collection that captures morphological changes and growth directions of spore buds under DMSO treatment conditions. The dataset spans the entire developmental timeline, covering approximately 90 to 210 minutes post-germination, with images acquired at 15-minute fixed intervals. This ensures that the dataset includes a complete morphological evolution sequence, from early germination stages to late developmental phases.